Lattice Light Sheet Microscopy

Lattice light sheet microscopy addresses this challenge by using thin sheets of light to illuminate the cell tissue or organism one slice at a time thus reducing the overall exposure to harsh laser light.

Lattice light sheet microscopy. Lattice light sheet microscopy is a newer light sheet microscopy technique that is primarily used in biological settings. It is a method that uses sheets of light in a lattice conformation to take images and videos of a sample by causing them to fluoresce. Lattice light sheet microscopy is a recent development with great potential to improve axial resolution and usable field sizes thus improving imaging speed. However due to the current limitations of llsm mechanism and the optical design of lls microscopes it is challenging to image large expanded specimens at isotropic high spatial resolution using llsm.

As a result the technique is gentle on live samples and has very. With lattice light sheet microscopy llsm scientists have been able to closely examine individual cells such as t cells in 4d. Lattice light sheet microscopy llsm was developed to image biological specimens rapidly at high 3d spatial resolution by using a thin lattice light sheet for sample illumination. The system generates an optical lattice to create an ultra thin light sheet to image biological samples over long periods of time and with very fine resolution.

The technology can visualize cellular processes in spatiotemporal detail not attainable with other fluorescent microscopy techniques. Lattice light sheet microscopy an ultrathin structured light sheet blue green center excites fluorescence orange in successive planes as it sweeps through a specimen gray to generate a 3d image. But with limited data points there wasn t an effective way to. Lattice light sheet microscopy is a modified version of light sheet fluorescence microscopy that increases image acquisition speed while decreasing damage to cells caused by phototoxicity this is achieved by using a structured light sheet to excite fluorescence in successive planes of a specimen generating a time series of 3d images which can provide information about dynamic biological.

Eric betzig the 3i lattice lightsheet microscope is capable of imaging biological systems spanning four orders of magnitude in space and time. The lattice light involves use of a bessel beam to illuminate a sample with light sheets that are sufficiently thin to achieve isotropic 3d resolution high speed 3d fluorescent imaging.